Coding

Part:BBa_K863034:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-18)

pcil35 laccase cDNA sequence from Pycnoporus cinnabarinus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 463
    Illegal NheI site found at 811
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1381


Design Notes

Primers for isolation of the cDNA sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagATGTCGAGGTTCCAGTCCCTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATCAGAGGTCGCTGGGGTCAA-3'

Source

The gene sequence was originally isolated from Pycnoporus cinnabarinus.

References